Quick Overview for Human IgG Isotype Control (ABIN1043618)
Target
Fragment
Application
Isotype
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Host
- Human
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Supplier Product No.
- 009-0105
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Supplier
- Rockland
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Purpose
- Human IgG Fab Fragment
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Cross-Reactivity (Details)
- Human IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti- Human IgG and anti- Human IgG F(ab')2. No reaction was observed against anti- Human IgG F(c) or anti-Papain.
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Characteristics
- Human Immunoglobulin Fab, F(ab), Fragment antigen-binding
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Purification
- Human IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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Application Notes
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Application Note: Human IgG Fab Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2.1 mg/mL
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Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.
Target
-
